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Effects of H and mH on NO production in LPS-induced RAW 264.7. Cells were cultured in the presence of H and mH for 1 h and stimulated with LPS (1 μg/mL) for 24 h under serum-free conditions. NO production was determined using Griess reagent. Nitrite concentrations of nontreated and LPS-treated controls were 4.63 ± 1.48 μM and 30.35 ± 0.12 μM, respectively. Each determination was made in triplicate. Data are presented as means ± SD. * p < 0.05 vs. LPS-treated group.
