Table 1. The parameters used for the detection of MANT fluorescence.
| Categories | Parameters | Values |
|---|---|---|
| Plate | Plate definition | [GRE384sb]-Greiner 384 Flat Black |
| Kinetic cycles | Number of cycles |
10 (Nucleotide loading assay) or 2,000 (Nucleotide exchange assay) |
| Kinetic interval | 15 sec | |
| Shaking | Duration | 5 sec |
| Mode | Orbital | |
| Amplitude | 3 mm | |
| Frequency | 217.3 rpm | |
| Fluorescence Intensity | Excitation wavelength | 360 ± 10 nm |
| Emission wavelength | 440 ± 20 nm | |
| Detection from | Top | |
| Gain (0-255) | 80 | |
| The number of Flash (400 Hz) | 8 | |
| Z-position | 24,012 µm |