Table 2. An example of the data obtained from loading of MANT-GDP on GTPase.

	0 sec	15 sec	30 sec	45 sec	60 sec	75 sec	90 sec	105 sec	120 sec	135 sec	Average	Normalized
Chlamydomonas Rabl2 (11.4 µM)	2,792	2,729	2,760	2,828	2,757	2,709	2,705	2,720	2,745	2,766	2,751.1	2,685.5
MANT-GDP (40 µM)	52,067	51,551	51,727	51,208	51,800	51,723	51,333	51,236	51,764	51,289	51,569.8	51,504.2
MANT-GDP (20 µM)	24,902	24,844	24,600	24,682	24,824	24,822	24,435	25,051	24,705	24,351	24,721.6	24,656
MANT-GDP (10 µM)	12,562	12,763	12,738	12,763	12,891	12,741	12,762	12,712	12,869	12,693	12,749.4	12,683.8
MANT-GDP (5 µM)	5,952	5,964	5,929	5,935	5,995	6,050	6,029	6,037	5,969	5,942	5,980.2	5,914.6
MANT-GDP (2.5 µM)	2,930	2,922	2,890	2,985	2,905	2,971	2,943	2,906	2,891	2,901	2,924.4	2,858.8
MANT-GDP (1.25 µM)	1,533	1,537	1,553	1,528	1,517	1,557	1,522	1,524	1,512	1,521	1,530.4	1,464.8
MANT-GDP (0.625 µM)	822	813	813	817	807	809	812	799	808	826	812.6	747
MANT-GDP (0.3125 µM)	428	423	421	419	421	424	420	420	422	419	421.7	356.1
MANT-GDP (0.15625 µM)	241	236	239	235	238	234	233	232	230	231	234.9	169.3
reaction buffer	67	64	67	66	66	64	68	65	65	64	65.6	0
The signal corresponds to free MANT-GDP	2.336
Estimated MANT-GDP concentration (µM)	1.168
Mol concentration of protein (µM)	11.4
Loading efficiency (%)	10.25