1 M Tris-HCl (pH 7.5)	12.5 ml	25 mM
5 M NaCl	15 ml	150 mM
1 M MgCl2	2.5 ml	5 mM
1M DTT	0.5 ml	1 mM
Triton X-100	0.5 ml	0.1%
0.25 M EDTA	2 ml	1 mM
Milli-Q water	467 ml