Volume	Final concentration
1 M HEPES-NaOH (pH 7.5)	12.5 ml	25 mM
5 M NaCl	5 ml	50 mM
1 M MgCl2	2.5 ml	5 mM
1M DTT	0.5 ml	1 mM
Glycerol	50 ml	10%
Milli-Q water	429.5 ml