| Volume | Final concentration | |
|---|---|---|
| 1 M HEPES-NaOH (pH 7.5) | 4 ml | 20 mM |
| 5 M NaCl | 2 ml | 50 mM |
| 1 M MgCl2 | 100 µl | 0.5 mM |
| Milli-Q water | 193.9 ml |
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