| Volume | Final concentration | |
|---|---|---|
| 1 M HEPES-NaOH (pH7.5) | 4 ml | 40 mM |
| 5 M NaCl | 1 ml | 50 mM |
| 1 M MgCl2 | 1 ml | 10 mM |
| 1 M DTT | 200 µl | 2 mM |
| Milli-Q water | 93.8 ml |
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