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. 2018 Jun 19;12:414. doi: 10.3389/fnins.2018.00414

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Copyright © 2018 Zheng, Shi, Liang, Xu, Li, Gao, Sun, Yu and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sirt3 knockdown abolished the positive effects of HKL and exacerbated cerebral injury. (A) Sirt3 expression. (B) NRF1 expression. (C) TFAM expression. ^∗P < 0.05 versus the rest three groups, ^#P < 0.05 (n = 6 in each group). (D) Ac-SOD2/SOD2 ratio. (E) Cleaved caspase3 expression. ^∗P < 0.05 versus the rest two groups, ^#P < 0.05 versus the rest four groups (n = 6 in each group). (F) Representative co-labeling TUNEL/NeuN pictures of ipsilateral basal ganglia. Quantification of TUNEL-positive neurons (the index of apoptosis = TUNEL-positive neurons/total neurons, n = 6 in each group), Scale bars: 100 μm. (G) Brain water content. (H) Neurologic deficit scores. (I) ROS levels. (J) ATP levels ^∗P and ^#P < 0.05 versus the rest groups (n = 6 in each group).