Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jun 19;9:830. doi: 10.3389/fpls.2018.00830

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 Colanero, Perata and Gonzali.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

The transient expression of SlMYB-ATV represses both SlDFR and its own promoters. Dual luciferase assays in MicroTom leaf protoplasts transfected with the reporter plasmid containing (A) the SlDFR or (B) the SlMYB-ATV promoter driving firefly luciferase (FireflyLuc) gene alone (basal level histogram) or in combination with effector plasmids containing SlAN2, SlAN1, SlJAF13, SlMYB-ATV, and Slmyb-atv sequences, in different combinations. A 35S:Renilla-luciferase (RenillaLuc) plasmid was used as an internal control. Data are expressed as Relative Luciferase Activity (RLU) (FireflyLuc/RenillaLuc) and are means of four biological replicates ± SE. One-way ANOVA test (Tukey’s multiple comparisons test, P < 0.05) was carried out. Different letters indicate significant differences in each bar chart plot. Each experiment was repeated at least three times with similar results.