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. 2018 Jun 19;9:830. doi: 10.3389/fpls.2018.00830

FIGURE 9.

FIGURE 9

SlMYB-ATV can interact with tomato bHLH factors involved in anthocyanin synthesis. Split-luciferase assays in MicroTom leaf protoplasts expressing the fusion proteins NLuc-SlMYB-ATV or NLuc-Slmyb-atv with CLuc-SlAN1 (A) or CLuc-SlJAF13 (B). As a control, NLuc-half protein was expressed in combination with each of the two CLuc-SlAN1 (A) or CLuc-SlJAF13 fusion proteins (B). Data are expressed as Relative Luciferase Activity (RLU) (RenillaLuc/protein content) and are means of four biological replicates ± SE. One-way ANOVA test (Tukey’s multiple comparisons test, P < 0.05) was carried out. Different letters indicate significant differences in each bar chart plot. Each experiment was repeated twice with similar results.