Table 2.
Reduced tiamulin and valnemulin susceptibility predicted by genome sequence based on the presence of mutations in chromosomal genes or the presence of tva(A).
| Tiamulin | Valnemulin | |||
|---|---|---|---|---|
| Chromosomal SNPs | tva(A) | Chromosomal SNPs | tva(A) | |
| Sensitivity | 77% | 95% | 68% | 100% |
| Specificity | 87% | 73% | 83% | 100% |
| Positive predictive value | 89% | 84% | 89% | 100% |
| Negative predictive value | 72% | 92% | 56% | 100% |
| True positive | 17 | 21 | 17 | 25 |
| True negative | 13 | 11 | 10 | 12 |
| False positive | 2 | 4 | 2 | 0 |
| False negative | 5 | 1 | 8 | 0 |
Isolate genotypes and susceptibilities described in Table 1. Specificity, sensitivity, and the predictive value of a positive and negative test were calculated using two-by-two table analysis (Mackinnon, 2000) with the following criteria: SNP/tva(A) presence and MIC > ECOFF value were true positive (TP), wild type SNP and MIC ≤ ECOFF value were true negative (TN), SNP/tva(A) presence but MIC ≤ ECOFF value were false positive (FP), and wild type SNP but MIC > ECOFF value were false negative (FN). Values ≥ 80% are indicated in bold font.