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Detection of deleted mtDNA genome with long-extension PCR (LX-PCR) and short extension PCR (SX-PCR). Total DNA from ragged red sections of fiber numbers 18–22 (lanes 1–5) was analyzed with LX-PCR and SX-PCR. (A) Nested PCR (25 + 25 cycles) with whole mtDNA genome LX-PCR amplification. (B) SX-PCR amplification (35 cycles) with F5881 and R15144 primers.
