Figure 5.

Co-localization of mtDNA deletion with segmental ragged red phenotype. COX and SDH enzymatic staining was performed on muscle fibers from a 38-month-old rat. ‘X’ (A and B) denotes the same fiber that shows normal COX activity in section (A) and negative COX phenotype in section (B). The distance between sections (A) and (B) is 550 µm. (C) The whole mtDNA genome PCR from ETS normal and ragged red sections of the same fiber: a, amplification of ETS normal section; b, amplification of ragged red section; M, DNA marker.