Wild-type mtDNA genome is not detectable in ragged red region. Deletion-specific primer set [5881 and 15144, with deletion products ∼1.3 kb (breakpoint 6349/14219)] and wild-type-specific primer set (14165 and 15144, with wild-type products ∼1.0 kb) were used to amplify deleted genomes and wild-type genomes, respectively, from ragged red and ETS normal sections of fiber number 32. Lanes 1 and 3, amplification with deletion-specific primer set; lanes 2 and 4, amplification with wild-type-specific primer set. WT, ETS normal section; RRF, ragged red section; M, DNA marker.