Skip to main content
. 2018 Jun 19;12:405. doi: 10.3389/fnins.2018.00405

FIGURE 5.

FIGURE 5

The protein levels of pmTOR, LC3B, P62, and HIF1α. (A) The protein bands of pmTOR, LC3B, P62, and β-actin detected using Western blotting and exposed by Tanon 2500/2500R. (B) The relative densities of LC3II/LC3I, (C) P62/β-actin, and (D) pmTOR/β-actin in the PMCAO group assessed in comparison with the SHAM group (∗p < 0.05, ∗∗∗p < 0.001), the AMCAO group vs. the PMCAO group (#p < 0.05, ###p < 0.001), and in the AMCAO group vs. the 3MAMCAO group (&p < 0.05, &&p < 0.01). The mean ± SEM of LC3II/LC3I and pmTOR/β-actin was calculated by one-way analysis of variance (ANOVA) followed by the least significant difference (LSD) post hoc test (homogeneity of variance was determined), while the mean ± SEM of P62/β-actin was calculated by one-way ANOVA followed by Tamhane’s T2 test (homogeneity of variance was not determined), N = 3. (E) The protein bands of HIF1α and β-actin detected using Western blotting and exposed by Tanon 2500/2500R. (F) The relative density of HIF1α/β-actin in the PMCAO group assessed in comparison with the SHAM group (∗∗p < 0.01, ∗∗∗p < 0.01), the AMCAO group vs. the PMCAO group (###p < 0.001) and the AMCAO group vs. the 3MAMCAO group (&&&p < 0.001). The mean ± SEM of HIF1α/β-actin was calculated by one-way ANOVA followed by the LSD post hoc test (homogeneity of variance was determined), N = 3.