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. 2018 Jun 25;8(7):62. doi: 10.1038/s41408-018-0098-z

Fig. 1. NS-018 suppresses IL-6-induced STAT3 phosphorylation and the proliferation of myeloma cell lines.

Fig. 1

a Docking models. The backbone of JAK2 is shown in green (left panel), the backbone of Src kinase in cyan (right panel), and NS-018 in pink (both panels). For clarity, only the amino acid side chains of the DFG motif are shown. b NIH3T3/v-Src cells were incubated with the indicated concentrations of NS-018 for 30 min. Cells were harvested and lysed with cell lysis buffer and the lysates analyzed by western blotting. c Myeloma cells were incubated with or without 5 ng/mL IL-6 for 2 h. The cells were harvested and lysed and the lysates analyzed for STAT3 and p-STAT3 by western blotting. d PCM6, U266, and RPMI 8226 cells were incubated with 10 ng/mL IL-6 for 2 h and then treated with the indicated concentrations of NS-018 for 30 min. The cells were harvested and analyzed for STAT3 and p-STAT3 as described above. e PCM6 cells were incubated with or without 10 ng/mL IL-6 and the indicated concentrations of NS-018 for 3 days and cell viability was analyzed by MTT assay