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. 2018 Jun 25;8:9646. doi: 10.1038/s41598-018-27821-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A,B) qRT-PCR determination of the intron-containing (unspliced) Chat, Chodl, Myh9 and Myh14 pre-mRNAs reveals a significant increase of the relative abundance of their unspliced pre-mRNAs in the spinal cords of SMN∆7 mice compared with WT mice at P14 (B). p values from WT and SMN∆7 data comparison at P14: 2.1E-3 for Chat, 3,5E-4 for Chodl, 6.3E-4 for Myh9 and 2.2E-4 for Myh14 (**p < 0.005; ***p < 0.0005). (A) During the late presymptomatic stage (P5), this increase was only detected in Chodl and Myh9 pre-mRNAs. p value from WT and SMN∆7 data comparison at P5: 2,1E-3 for Chodl and 3,5E-4 for Myh9 (**p < 0.005; ***p < 0.0005). No significant changes (n.s) in unspliced Actb pre-mRNAs were detected when samples from WT and SMN∆7 mice were compared at either P5 or P14. The bars represent the mean ± SD of the relative fraction of the total RNA transcript that is unspliced pre-mRNA from WT (n = 3) and SMN∆7 mice (n = 5). qRT-PCR analyses were always confirmed in triplicate.