Table 2.
Open reading frames and ITRs in the 29,342 bp genome of RaegAdV-3085.
| ORF number | Gene/protein designation or feature | Predicted description or function of protein | Structural or non-structural | Gene (nt) | Protein (aa) | Genomic position |
|---|---|---|---|---|---|---|
| ITR | Forms panhandles for replication | N/A | 40 | N/A | 1–40 | |
| 1 | E1A | Transccriptional activator | Non-structural | 516 | 171 | 456–971 |
| 2 | E1B small | Small T-antigen | Non-structural | 372 | 123 | 1,324–1,695 |
| 3 | E1B large | Large T-antigen | Non-structural | 1,173 | 390 | 1,755–2,927 |
| 4 | IX | Minor capsid protein | Structural | 249 | 82 | 2,942–3,190 |
| 5 | IVa2 | DNA packaging ATPase | Structural | 1,110 | 369 | 3,214–4,323 (c-strand) |
| 6 | pol | DNA polymerase | Non-structural | 2,922 | 973 | 4,311–7,232 (c-strand) |
| 7 | pTP | Terminal protein | Structural | 1,794 | 597 | 7,568–9,361 (c-strand) |
| 8 | 52 K | DNA packaging protein | Non-structural | 1,014 | 337 | 9,415–10,428 |
| 9 | pIIIa | Minor capsid protein | Structural | 1,566 | 521 | 10,382–11,947 |
| 10 | Penton | Major capsid protein | Structural | 1,425 | 474 | 12,029–13,453 |
| 11 | pVII | Major core protein | Structural | 390 | 129 | 13,459–13,848 |
| 12 | V | Minor core protein | Structural | 1,101 | 366 | 13,929–15,029 |
| 13 | pX (µ) | Minor core protein | Structural | 198 | 65 | 15,084–15,281 |
| 14 | pVI | Minor capsid protein | Structural | 600 | 199 | 15,324–15,923 |
| 15 | Hexon | Major capsid protein | Structural | 2,727 | 908 | 15,988–18,714 |
| 16 | AVP | Protease | Structural | 603 | 200 | 18,943–19,545 |
| 17 | DBP | DNA binding protein | Non-structural | 1,260 | 419 | 19,583–20,842 (c-strand) |
| 18 | 100 K | Hexon scaffold protein | Non-structural | 2,049 | 682 | 20,853–22,901 |
| 19 | 33 K | DNA packaging/assembly protein | Non-structural | 291 | 96 | 23,087–23,377 |
| 20 | pVIII | Minor capsid protein | Structural | 585 | 194 | 23,428–24,012 |
| 21 | E3 14.7 K | Immuno-modulatory protein | Non-structural | 372 | 123 | 24,016–24,387 |
| 22 | U exon | Replication centre protein | Non-structural | 168 | 55 | 24,399–24,566 (c-strand) |
| 23 | Fiber protein | Major capsid protein | Structural | 1,773 | 590 | 24,565–26,337 |
| 24 | E4 ORF34K | p53 and p73 inhibitor | Non-structural | 738 | 245 | 26,368–27,105 (c-strand) |
| 25 | Hypothetical protein | Unknown | Unknown | 309 | 102 | 27,118–27,426 (c-strand) |
| 26 | Hypothetical protein | Unknown | Unknown | 396 | 131 | 27,420–27,815 (c-strand) |
| 27 | dUTP pyrophosphatase | pyrophosphatase | Non-structural | 393 | 130 | 27,809–28,201 (c-strand) |
| ITR | Form panhandles for replication | N/A | 40 | N/A | 29,303–29,342 |