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. 2018 Jun 25;8:9645. doi: 10.1038/s41598-018-27927-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of TNF on catecholaminergic differentiation marker expression. Immunofluorescent detection of tyrosine hydroxylase (TH, key enzyme of catecholamine synthesis), vesicular monoamine transporter 2 (VMAT2), nuclear receptor related 1 protein (Nurr1), and β3-tubulin in OA and RA adipose tissue-derived stem cells undergoing catecholaminergic differentiation treated with 10 ng/ml TNF and/or 1 or 10 µg/ml etanercept (ETA) in different concentrations and combinations (magnification 200x).