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. 2018 Jun 19;9:1412. doi: 10.3389/fimmu.2018.01412

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Copyright © 2018 Wefers, Schreibelt, Massuger, de Vries and Torensma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cancer stem cells in high-grade serous ovarian cancer ascites. (A) Isolated mononuclear cells from ascites of ovarian cancer patients (n = 13) were stained for CD45, NANOG, and HLA-ABC. NANOG positivity was assessed in the CD45− population to exclude immune cells. NANOG− and NANOG+ cells were gated and HLA-ABC expression was analyzed in both populations. Gray line = isotype control; black line = sample. (B) Percentage of NANOG+ cells in ascites. (C) Percentage of HLA-ABC positive cells in the NANOG− and NANOG+ population. Red line indicates mean.