Figure 6.

Structure probing of 5′-end-labeled wild-type and U7445C tRNA^Ser(UCN) precursors. Precursor tRNA^Ser(UCN) prepared using the double ribozyme method was labeled at its 5′-end and incubated at room temperature for 5 min with increasing concentrations of RNase T1 (0.5, 1 and 2 U/ml) or RNase A (2, 4 and 8 × 10^–3 U/ml) as indicated. RNase T1 and RNase A bands are identified by nt number at right. (A) Wild-type; (B) U7445C. (C) Susceptibility of the wild-type and U7445C tRNA precursors to structure probing nucleases. Solid and dashed arrows designate sites susceptible to RNase T1 and A, respectively.