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. 2018 Jun 19;9:753. doi: 10.3389/fphys.2018.00753

FIGURE 4.

FIGURE 4

Immunohistochemistry indicates HCN channel expression on smooth muscle tissue. (A) Van Gieson staining of a human bladder specimen (patient #2 in Table 2) demonstrates the presence of smooth muscle tissue. (B–D) The immunohistochemical labeling of HCN1, 2 and 4 suggests that all these isoforms are present on smooth muscle tissue. Scale bar 100 μm.