Figure 4.

Northern blot analysis detects CeABF-1 expression. The expression pattern of CeABF-1 in nematodes was analyzed from pooled organisms found at the embryo, larval and adult stages. Ten micrograms of total RNA was isolated and analyzed by northern blot analysis. As a control for size markers, HeLa cells were transfected with an inducible cloning vector carrying full-length human ABF-1. HeLa cells were either induced with tetracycline (+) or not induced (–). Total RNA was prepared from the HeLa cells and examined by northern blot analysis on the same gel. Following hybridization with either the human ABF-1 probe or CeABF-1 probe, the membranes were aligned and the human transcript used as size markers. The asterisk designates the detected CeABF-1 transcript.