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CeABF-1 binds as a heterodimer to an E-box in vitro. EMSA analysis of human ABF-1 and CeABF-1 heterodimeric complexes formed on the µE5 probe. Lines indicate the position of E47–ABF-1 and E47–CeABF-1 heterodimers. The symbol (co) indicates the proteins E2A and ABF-1 or CeABF-1 were cotranslated. Addition of an antibody specific to E47 or the FLAG-tagged ABF-1 is indicated by the labeling αE47 and αFLAG, respectively.
