Figure 7.

CeABF-1 functions as a transcriptional repressor in mammalian cells similar to human ABF-1. HeLa S3 cells were transiently transfected with 1 µg of (µE5-µE3-µE2)₄-Luc reporter plasmid and different combinations of either the E47 expression vector (0.5 µg) with expression vector FLAG-human ABF-1, FLAG-CeABF-1, CeABF-1 N-73 or CeABF-1 C-97 (0.25 µg). Twenty-five microliters of Superfect reagent was used for each transfection. Equal amounts of protein extract were assayed for firefly luciferase activity. Experiments were performed in triplicates and run at least three independent times. Luciferase activity was normalized to β-galactosidase levels as a control for transfection efficiency.