Table 1. (Photo)cytotoxicity (EC50 with confidence interval (95%) in μM) of [1]Cl, [2]PF6 and cisplatin on skin (A431) and lung (A549) cancer cell lines given with photo index (PI). a , b In addition, the complexes were tested against a non-cancerous lung cell line (MRC-5) for comparison.
| Cell line | t incubation (h) | Light dose (J cm–2) | [1]Cl |
[2]PF6 |
Cisplatin |
||||||
| EC50 (μM) | ±CI (95%) | PI | EC50 (μM) | ±CI (95%) | PI | EC50 (μM) | ±CI (95%) | PI | |||
| A431 | 6 | 0 | 13.0 | 1.30 | 22 | 38.0 | 8.8 | 4.9 | 4.3 | 1.5 | 0.9 |
| 75 | 0.60 | 0.05 | 7.80 | 1.0 | 4.6 | 1.5 | |||||
| 24 | 0 | 10.0 | 0.59 | 11 | 30.0 | 4.3 | 2.1 | 4.8 | 1.6 | 1.0 | |
| 75 | 0.88 | 0.24 | 14.0 | 1.1 | 4.9 | 1.6 | |||||
| A549 | 6 | 0 | 9.30 | 2.30 | 16 | 20.0 | 6.1 | 5.6 | 3.3 | 0.55 | 1.0 |
| 75 | 0.58 | 0.08 | 3.60 | 1.0 | 3.3 | 0.54 | |||||
| 24 | 0 | 6.20 | 0.86 | 9.5 | 11.0 | 1.0 | 2.2 | 3.1 | 0.55 | 0.8 | |
| 75 | 0.65 | 0.0 | 5.00 | 0.4 | 3.6 | 0.77 | |||||
| MRC-5 | 6 | 0 | 13.0 | 1.3 | 8.1 | 8.50 | 3.5 | >8.5 | 3.8 | 1.5 | n.d c |
| 75 | 1.60 | 2.4 | <1 | n.d. c | |||||||
| 24 | 0 | 8.30 | 1.0 | 4.9 | 18.3 | 1.4 | >18 | 6.9 | 1.2 | n.d c | |
| 75 | 1.70 | 2.3 | <1 | n.d c | |||||||
a“Light” = green light irradiation (520 nm, 60 min, 75 J cm–2).
bIncubation time is the time the Ru complex is incubated (37 °C, 7% CO2) with cells in the dark before light irradiation.
cn.d. stands for not determined.