TABLE 1.
Validated isolates and reference strains and their sources
| Pathogen | Sourcea |
|---|---|
| Bovine viral diarrhea virus (strain Singer, type 1a) | A |
| Bovine viral diarrhea virus (strain 125, genotype 2) | A |
| Bovine herpesvirus type 1 (strain Colorado) | A |
| Bovine herpesvirus type 4 (DN-599) | A |
| Bovine coronavirus (strain Nebraska) | A |
| Bovine respiratory syncytial virus (strain A51908) | A |
| Influenza virus type D | B |
| Parainfluenza virus type 3 (strain SF-4) | A |
| Rotavirus A (strain Nebraska) | A |
| Bluetongue virus (serotype 10 tested) | A |
| Adenovirus type 3 | C |
| Mannheimia haemolytica | C |
| Trueperella pyogenes | C |
| Bibersteinia trehalosi | C |
| Histophilus somni | C |
| Mycoplasma spp. (Mycoplasma bovis tested) | C |
| Escherichia coli strains that possess Shiga toxin 1 (stx1), intimin (eae), alpha hemolysin (hlyA), cytotoxic necrotizing factor (cnf1 and cnf2), enterotoxin (STa), fimbrial (k99), and F41 virulence factor genes | D |
| Clostridium perfringens strains that possess α, β, β-2, enterotoxin (CPE), ε, and ι toxin genes; | C |
| Mycobacterium avium subsp. paratuberculosis (MAP) | C |
| Salmonella spp. | C |
| Campylobacter fetus subspecies (Campylobacter fetus subsp. fetus and Campylobacter fetus subsp. venerealis) | E |
| Listeria monocytogenes | C |
| Brucella spp. (B. canis tested) | C |
| Leptospira spp. (serovar Pomona tested) | A |
| Ureaplasma spp. (U. parvum tested) | F |
| Staphylococcus aureus | C |
| Coagulase-negative Staphylococcus (S. epidermidis tested) | C |
| Streptococcus agalactiae | C |
| Streptococcus dysgalactiae | C |
| Streptococcus uberis | C |
| Nocardia spp. (N. nova tested) | C |
| Pseudomonas spp. (P. aeruginosa tested) | C |
| Klebsiella spp. (K. pneumoniae tested) | C |
| Chlamydia spp. (C. felis tested) | C |
| Anaplasma marginale | C |
| Neospora caninum | G |
| Toxoplasma gondii | G |
| Tritrichomonas fetus | F |
| Cryptosporidium spp. (C. parvum tested) | C |
| Giardia intestinalis | C |
| Prototheca spp. (P. zopfii tested) | E |
| Aspergillus spp. (A. fumigatus tested) | C |
| Fusarium spp. | C |
A, USDA National Veterinary Services Laboratory; B, kindly provided by Henry Wan, Mississippi State University; C, detected in a diagnostic sample and identified/isolated in the TVDIL according to a validated protocol; D, E. coli Reference Center, Pennsylvania State College of Agriculture Sciences; E, validated reference kindly provided by Amy Swinford, Texas Veterinary Medical Diagnostic Laboratory; F, American Type Culture Collection, Manassas, VA; G, validated reference kindly provided by Chunlei Su and Rick Gerhold, University of Tennessee. TVDIL is accredited by the American Association of Veterinary Laboratory Diagnosticians (requirements are based on the ISO/IEC 17025 2005 standard General Requirements for the Competence of Testing and Calibration Laboratories).