TABLE 2.
Bacterial strains and plasmids used in the study
| Strain or plasmid | Genotype or relevant features | Source or reference |
|---|---|---|
| Strains | ||
| mc2155 | Wild-type strain of Mycobacterium smegmatis | ATCC |
| Δms33 | msmeg_2433 deletion strain of M. smegmatis; Hygr | This study |
| Δms32 | msmeg_2432 deletion strain of M. smegmatis; Hygr | This study |
| Δms33/32 | msmeg_2433/32 deletion strain of M. smegmatis; Hygr | This study |
| Δms33/32::pM33 | Δms33/32 complemented with msmeg_2433 (pM33); Hygr Kanr | This study |
| Δms33/32::pM32 | Δms33/32 complemented with msmeg_2432 (pM32); Hygr Kanr | This study |
| Δms33/32::pM11 | Δms33/32 complemented with dacB2/rv2911 (pM11); Hygr Kanr | This study |
| Δms33/32::pMIND | Δms33/32 transformed with empty vector pMIND; Hygr Kanr | This study |
| E. coli XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIq ZΔM15 Tn10 (Tetr)] | Stratagene |
| Plasmids | ||
| pSMT100 | Mycobacterial suicide vector; sacB Hygr | 40 |
| pSD33-2 | pSMT100 with right and left flanking regions of msmeg_2433; Hygr | This study |
| pMIND | Tetracycline-inducible vector; Kanr | Bose Institute, Kolkata, India |
| pM33 | pMIND harboring msmeg_2433; Kanr | This study |
| pM32 | pMIND harboring msmeg_2432; Kanr | This study |
| pM11 | pMIND harboring dacB2/rv2911 of M. tuberculosis; Kanr | This study |