FIG 3.
Export of EGFP by the Tat pathway with different RR signal peptides in H. pylori. The expression and localization of EGFP fused with different RR signal peptides were analyzed for H. pylori strains (G27/PcagA-HydA-EGFP, G27/PcagA-KapA-EGFP, G27/PcagA-KapA-Δtat-EGFP, and G27/PcagA-TorA-EGFP), indicated as HydA, KapA, KapA-Δtat, and TorA. Cells were fractionated to yield cytoplasmic (C) and periplasmic (P) samples and immunoblotted by using an antibody to GFP. Mobilities of molecular mass markers for 38 and 28 kDa are indicated.