TABLE 1.
Oligonucleotide primers used in this study
| Primer | Sequence (5′–3′)a | Siteb | Templatec |
|---|---|---|---|
| HP0545-5P(400-430)-KpnI | gggcgaattgggtaccTTTCTTGTCTTTCAAATTTTTG | KpnI | H. pylori |
| HP0547-3P(1785-1860) | TGTTTCTCCTTACTATACCTAG | H. pylori | |
| HP0547-5P(1808-1828) | ataataagcggatgaATGACTAACGAAACTATTGATC | H. pylori | |
| HP0547-3P(1542-1569)-SacI | caaaagctggagctcTTGGACATGGGGAACTGG | SacI | H. pylori |
| Ch-5 | gaattcctgcagAAATCCTGGTGTCCCTGTTG | pBC SK+ | |
| Ch-3 | TCATCCGCTTATTATCACT | pBC SK+ | |
| EGFP-5 | GTGAGCAAGGGCGAGGAGCTGTTCACCGG | pEGFP-C1 | |
| EGFP-3 | ctgcaggaattcTTACTTGTACAGCTCGTCCAT | pEGFP-C1 | |
| HydA-F | ctaggtatagtaaggagaaacaATGTTCTACGATGAAAAAAAGACC | H. pylori | |
| HydA-R | cagctcctcgcccttgctcacCGCCTTCAAAGTCAAGGGAGCAAAAC | H. pylori | |
| KapA-F | ctaggtatagtaaggagaaacaATGAAACGAAGGGATTTTATTAAAAC | H. pylori | |
| KapA-Δtat-F | ctaggtatagtaaggagaaacaATGACGACTACTTTAGGCGCTACAGGTGC | H. pylori | |
| KapA-R | cagctcctcgcccttgctcacTGCCTGCAAAATCTGTGCTCC | H. pylori | |
| TorA-F | ctaggtatagtaaggagaaacaATGAACAATAACGATCTCTTTCAGG | E. coli | |
| TorA-R | ctaggtatagtaaggagaaacaCGCCGCTTGCGCCGCAGTCGCAC | E. coli |
a
Sequences in uppercase type are derived from the genome sequences of H. pylori 26695 (51) and E. coli (32) and vector sequences. Sequences introduced for cloning purposes (end-terminal homology with the adjacent fragment) are in lowercase type, and restriction recognition sites are underlined.
b
Restriction recognition sites.
c
Genomic DNA from H. pylori strain G27 and E. coli Top10 and plasmid DNA from pBS SK+ and pEGFP-C1 served as templates for PCR.