Figure 4.

SNHG1 was targeted by miR-137 at 3′UTR. (A) The predicted sequence of miR-137 in the 3′UTR of SNHG1 is verified. (B) Relative luciferase report assay in 786-O cells co-transfected with miR-137 or NC and wild-type or mutant SNHG1 3′UTR. (C) miR-137 expression was measured by qRT-PCR assay following transfection with si-SNHG1 and NC. (D) Expression of miR-137 was identified in RCC tissues compared with adjacent normal tissues (P<0.001). (E) Patients with low level of miR-137 had better prognosis compared with those with high level in TCGA database (log-rank test, P=0.00067). (F) The correlation between SNHG1 and miR-137 levels was detected. Data are presented as mean ± standard error based on at least 3 independent experiments. * P<0.05 and ** P<0.01.