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. 2018 Jun 19;9:828. doi: 10.3389/fpls.2018.00828

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Copyright © 2018 Ma, Xu, Alejos-Gonzalez, Wang, Yang, Judd and Xie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of enzymatic activity with HPLC analysis and kinetic features. (A) HPLC profiles show conversion of coniferyl aldehyde to coniferyl alcohol, a-1: reaction product and substrate, a-2: coniferyl aldehyde standard, and a-3: coniferyl alcohol standard. (B) Profiles of HPLC show conversion of sinapyl aldehyde to sinapyl alcohol, b-1: reaction product and substrate, b-2: sinapyl aldehyde standard, and b-3: sinapyl alcohol standard. (C) Profiles of HPLC show conversion of p-coumaryl aldehyde to p-coumaryl alcohol, c-1: reaction product and substrate, c-2: p-coumaryl aldehyde standard, and c-3: p-coumaryl alcohol standard. (D) Km, Vmax, Kcat, and Vcat/Km values were calculated for three substrates. All experiments in A–C were repeated at least three times. All experiments in D were carried out with three replicates to calculate the values.