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. 2018 Jun 19;9:828. doi: 10.3389/fpls.2018.00828

FIGURE 3.

FIGURE 3

A simplified map of the T-DNA cassette and integration of the transgene in transgenic plants. (A) A T-DNA cassette constructed in a binary vector showing the use of the 2 × 35S promoter for overexpression of AaCAD in A. annua. (B) Images show phenotypes of an OE3 transgenic line vs. one wild-type control plant. (C) PCR gel images show the genomic DNA fragment amplified from two transgenic lines using a pair of primers consisting of a forward 35S promoter primer and a reverse CAD primer. (D) Gel images show the increased expression of AaCAD in transgenic plants. (E) Gel images show the increased AaCAD protein in transgenic lines. Abbreviations in T-DNA: AaCAD, Artemisia annua cinnamyl alcohol dehydrogenase; GFP, green florescent protein; Hyg, hygromycin; Tnos, Nos terminator. Plant name abbreviation: OE2 and OE3, two transgenic lines; WT, wild-type control. PCR and RT-PCR experiments were performed with three biological replicates, each with at least three replicates. Western blotting was repeated three times.