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. 2018 Jun 19;9:828. doi: 10.3389/fpls.2018.00828

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Copyright © 2018 Ma, Xu, Alejos-Gonzalez, Wang, Yang, Judd and Xie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of AaCAD overexpression on levels of artemisinin, arteannuin B, and other sesquiterpenes in leaves of transgenic plants. (A) LC-MS analysis measurements show decreases in artemisinin contents. (B) Overlay of total ion chromatographs shows the abundance reduction of numerous non-polar metabolites, among which arteannuin B and sesquiterpenes are labeled as follows: “IN”, internal metabolite as a control. (C) Peak area values show the significant reduction of arteannuin B. (D) Peak area values show the reduction of five sesquiterpenes. ^∗ in (A) and ^∗∗ in (C) and (D) denote a significant difference in the transgenic lines compared with WT plants. (N = 3, p < 0.01). All experiments were carried out with three biological replicates, each with three replicates.