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Numbers of adult SD rats used in the in vivo assay.
| Method | Number | |||||||
|---|---|---|---|---|---|---|---|---|
| pcDNA3.1(+)-7 d | pcDNA3.1(+)-Faslg-7 d | pcDNA3.1(+)-14 d | pcDNA3.1(+)-Faslg-14 d | NC-7 d | siRNA-Faslg-7 d | NC-14 d | siRNA-Faslg-14 d | |
| Real-time PCR | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 |
| Western blot | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 |
| Immunohistochemistry | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 |
| Sum | 288 | |||||||
The experiments were repeated three times. Abbreviations: NC, negative control; siRNA, short interfering RNA; Faslg, Fas ligand gene; PCR, polymerase chain reaction.
