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. 2018 Jun 25;8:9596. doi: 10.1038/s41598-018-27943-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Spatial association of endothelial cells and pericytes. (A,B) Association in the angiogenic front region. (A) Endothelial tip cells (arrow) and pericytes (asterisk) association at the angiogenic front. (B) Quantification of pericyte – endothelial tip cell distance in the front region. (C–E) Association in the established region. (C,D) Close to the endothelial tip cell (arrow) pericytes are found in the branching points (white arrowhead) or around the tube (yellow arrow). (E) Quantification of pericyte – endothelial tip cell distance in the established vessel region. Data represents mean ± SEM. *p < 0.05 versus (B). Scale bars represent 100 µm.