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. 2018 Jun 25;8:9596. doi: 10.1038/s41598-018-27943-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Retrospective analysis of pericyte detachment and recruitment. Images of vessel development taken at T = 0, 6, 12 and 24 hrs were re-evaluated. (A) Subsequential projection of a developing endothelial sprout (arrow) and an adjacent dispersing (white arrowhead) and re-emerging (yellow arrowhead) pericyte. (B) Forward movement of endothelial cells (arrow) and recruitment of pericytes (arrowhead) during anastomosis. Blood flow is detected using pegylated nanoparticles (PEG-NP) that remain in the blood circulation for approximately 20 hrs. Scale bars represent 50 µm.