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. 2018 Jun 25;8:9596. doi: 10.1038/s41598-018-27943-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Re-evaluating pericytes using whole-mount staining. (A,B) Re-evaluating spatial endothelial cell – pericyte association. (A) Intravital images of Cspg4+ pericytes shown in Fig. 4 re-evaluated after desmin staining (arrow). (B) Cspg4+ pericytes re-stained with NG2 (arrowheads) shown in close proximity of endothelial tip cells (arrow). (C–E) Co-localization of pericyte markers. (C,D) Whole-mount staining of tumor tissue showed Pdgfrb+ or Cspg4+ pericytes co-expressing desmin (white arrow), Pdgfrb+ pericytes not stained for desmin (arrowhead), and Pdgfrb- or Cspg4- pericytes stained positive for desmin (yellow arrow). (E) Quantification of co-expression of Pdgfrb or Cspg4 with desmin. Data represent mean ± SEM. Scale bars represent 50 µm.