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. 2018 Jun 25;8:9630. doi: 10.1038/s41598-018-27719-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Intra-lineage analysis of differentially expressed miRNAs. (A–C) Hierarchical clustering of 170 differentially expressed small RNAs in the first ten days of HD, 177 differentially expressed small RNAs of KD, and 1,040 differentially expressed small RNAs of ND, respectively. The values of two biological replicates (indicated in color) for each time-point are shown. (D–F) The miRNAs with differential dynamics changes were plotted in different colors: small RNAs that decreased with differentiation (red); small RNAs enriched in stage 1 and 4 but downregulated at stage 2 (yellow); small RNAs increased at stage 2 but decreased at later stages (green); small RNAs enriched in stage 3 (pink); and small RNAs only increased at the latest stage (blue).