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. 2018 Jun 25;8:9630. doi: 10.1038/s41598-018-27719-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Regulation of lineage-specific gene expression by lineage-specific miRNAs during hepatocyte differentiation. (A) qPCR results showing the expression of definitive endoderm markers GATA4 and GATA6 in HD 6 cells with transfection of miR-192-3p/5p mimics (n = 3 independent cultures for each group). (B) qPCR results showing the expression of intermediate mesoderm marker PAX2 and metanephric mesenchyme marker HOXD11 in HD 12 cells upon transfection of miR-192-3p/5p inhibitors (n = 3 independent cultures for each group). (C) HD 12 cells were examined by indocyanine green staining (ICG). The cells transfected with miR-192 mimics (left column) and scramble controls (right column) were compared (Scale bars represent 100 μm). (D) Table showing the percentage of ICG⁺ cells at HD 12 with the transfection of miR-192 mimics and non-targeting controls (data are presented as the means ± SD. n = 3 independent cultures for each group). In (A,B), data are presented as the means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 for statistical comparisons between control groups and experimental groups (ANOVA plus Bonferroni’s post hoc test). OE: overexpression; KnD: Knockdown; NC: non-targeting control.