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. 2001 Nov 1;29(21):4493–4501. doi: 10.1093/nar/29.21.4493

Figure 2.

Figure 2

MeCP2 represses transcription from a methylated L1 promoter. (A) Schematic representation of the sites (nucleotides 36, 101, 304 and 481) in the L1.3–5′UTR methylated by M.HpaII methylase. (B) HEK293 cells were co-transfected with unmethylated (U) or HpaII-methylated (Me) reporter L1.3-Luc and expression constructs encoding full-length FLAG-tagged MeCP2, MBD1v3 or MBD2b. Relative luciferase activity of the unmethylated reporter in the absence of coexpressed genes was set as 1.0. Columns represent mean luciferase activities (± standard deviations) of three to five independent experiments. (C) Expression of FLAG-tagged MeCP2, MBD1v3 and MBD2b was controlled for by immunoblot analysis with an anti-FLAG monoclonal antibody. The tagged proteins exhibit apparent molecular masses of 81, 81 and 29 kDa, respectively.