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. 2001 Nov 1;29(21):4493–4501. doi: 10.1093/nar/29.21.4493

Figure 4.

Figure 4

Methylated and overexpressed full-length MeCP2 diminishes L1 retrotransposition frequency. (A) Unmethylated (U) or HpaII-methylated (Me) reporter JM101/L1.2ΔCMV was co-transfected with empty vector pcDNA1.1 or with the expression construct for FLAG-tagged MeCP2. Results of a representative transposition assay are shown. (B) Effect of MeCP2 on relative retrotransposition frequencies of the methylated versus unmethylated neo-marked L1.2 reporter. The bar chart is based on the data in Table 1B. Relative retrotransposition frequencies refer to the mean retrotransposition rate of the unmethylated reporter JM101/L1.2ΔCMV (612 ± 40 × 106), which is set as 1.0. (C) Expression of FLAG-tagged MeCP2 (81 kDa) 72 h post-transfection was controlled for by immunoblot analysis with anti-FLAG antibody.