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. 2018 Jun 25;9:2459. doi: 10.1038/s41467-018-04883-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Serotonergic dysfunction in Eif4e ki/ki mice. a Representative whole cell recordings of spontaneous excitatory post-synaptic currents (sEPSCs) from layer V pyramidal neurons of the medial prefrontal cortex (mPFC) of wild-type and Eif4e ki/ki mice. Traces represent consecutive recordings of synaptic currents before (baseline) and after 5-HT (20 μM) treatment from single pyramidal cells in each group. b Frequency of sEPSCs from pyramidal neurons of wild-type and Eif4e ki/ki mice before (−) and after 5-HT at 20 μM (n = 7 cells from 5 Eif4e^+/+; n = 8 cells from 7 Eif4e ki/ki), 50 μM (n = 4 cells from 3 Eif4e^+/+; n = 5 cells from 3 Eif4e ki/ki) or 100 μM (n = 5 cells from 3 Eif4e^+/+; n = 6 cells from 3 Eif4e ki/ki). c In vivo single-unit extracellular recordings of dorsal raphe neurons were performed in anesthetized Eif4e^+/+and ki/ki mice. d Firing rate of dorsal raphe 5-HT neurons, measured in Eif4e^+/+and ki/ki mice (n = 56 single unit recordings from 8 Eif4e^+/+mice; n = 43 single unit recordings from 7 Eif4e ki/ki mice). e 5-HT neurons recorded per descent (track) in the dorsal raphe of wild-type and Eif4e ki/ki mice (n = 8 Eif4e^+/+mice; n = 7 Eif4e ki/ki mice). f Firing rate of non-5-HT neurons in the DR (n = 27 single unit recordings from 6 Eif4e^+/+mice; n = 16 single unit recordings from 5 Eif4e ki/ki mice). g Number of non-5-HT neurons per descent in the DR (n = 6 Eif4e^+/+mice; n = 5 Eif4e ki/ki mice). h Number of NeuN + cells in the DR nuclei, which was subdivided into DR dorsal part (DRD), DR lateral part (DRL), posterodorsal raphe nucleus (PRN), and DR ventral part (DRV). Data are means of 4 slides per animal (n = 4 Eif4e^+/+mice; n = 5 Eif4e ki/ki mice). i Number of tryptophan hydroxylase 2 (TPH2) positive cells in the DRN. j Representative images of the DR IHC; blue: nuclear DAPI staining; red: TPH2; green: NeuN. Scale bar is 100 μM. * p < 0.05, *** p < 0.001 vs. wild-type littermates (see Supplementary Table 2 for detailed results of statistical tests)