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. 2018 Jun 25;9:2459. doi: 10.1038/s41467-018-04883-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — TNFα causes serotonergic impairments in Eif4e ki/ki mice. a Representative sEPSC traces from mPFC pyramidal neurons in brain slices of wild-type mice treated with vehicle (saline), DN TNF (200 ng ml⁻¹) or mouse recombinant TNFα (10 ng ml⁻¹). Synaptic currents were recorded before (baseline) and after 5-HT (20 μM) administration. b Representative sEPSC traces from Eif4e ki/ki mice treated with either vehicle or DN TNF (200 ng ml⁻¹), measured before and after 5-HT (20 μM) administration. c Frequency of sEPSCs in mPFC slices of male and female wild-type mice. Slices were directly treated with saline (M: n = 10 cells from 6 mice; F: n = 9 cells from 6 mice), DN TNF (200 ng ml⁻¹; M: n = 8 cells from 5 mice; F: n = 7 cells from 4 mice) or mouse recombinant TNFα (10 ng ml⁻¹; M: n = 9 cells from 6 mice; F: n = 6 cells from 5 mice), pre- and post-5-HT (20 μM) application. d Average sEPSC frequency in Eif4e ki/ki mice before and after 5-HT (20 μM) application; slices were treated with either saline (M: n = 11 cells from 6 mice; F: n = 9 cells from 7 mice) or DN TNF (200 ng ml⁻¹; M: n = 8 cells from 6 mice; F: n = 9 cells from 7 mice). e, Firing rate of DR neurons in wild-type and Eif4e ki/ki mice chronically treated with DN TNF or saline (ICV, 12 days; n = 33 recordings from 6 Eif4e^+/+Saline mice; n = 31 recordings from 5 Eif4e^+/+DN TNF mice; n = 16 recordings from Eif4e ki/ki Saline mice; n = 33 recordings from 6 Eif4e ki/ki DN TNF mice). * p < 0.05, ** p < 0.01, *** p < 0.001 vs. wild-type saline; & p < 0.05, && p < 0.01, &&& p < 0.001 vs. saline-treated Eif4e^+/+or ki/ki (see Supplementary Table 2 for detailed results of statistical tests)