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. 2018 Jun 19;12:45. doi: 10.3389/fnana.2018.00045

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Copyright © 2018 Horn, Horng, Buresch, Messoudi and Härtig.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A,B) Detailed microphotographs of sections from Figure 1 showing the monkey nVI stained for ChAT (black) and CSPG (brown). ChAT-positive neurons lacking PNs represent motoneurons of MIF (open arrow) (A), those enwrapped by PNs represent motoneurons of singly-innervated muscle fibers (thin arrows) (A,B), and the ChAT-negative ones INTs (B, star). (C,D,E) Image from double immunoflourescence in nVI of a monkey that had received a wheat germ agglutinin (WGA) injection into the oculomotor nucleus to backlabel INTs shown in (C) (green). Detection of aggrecan (ACAN) revealed that INTs (C, small arrows) are enwrapped by PNs (D, small arrows), more clearly seen in the overlay (E, arrows). The large arrow indicates a WGA-negative neuron enwrapped by a PN, which could represent a motoneuron. Scale bar = 30 μm in (A; applies to A,B); 30 μm in (C; applies to C–E).