Table 1.
Case | Tract-Tracer | Fixation | Cutting | Staining for light microscopy | Staining for fluorescence microscopy |
---|---|---|---|---|---|
M1 | 4% PFA | paraffin | ChAT (DAB-Ni) + CSPG (DAB) | ||
M2 | CTB in nIII | 4% PFA | frozen | CTB | CTB + ChAT |
M3 | WGA in nIII | 4% PFA | frozen | ACAN + WGA | |
M4 | 4% PFA | frozen | AChE enzyme histochemistry | ||
M5 | 4% PFA | frozen | ChAT, Nissl | ||
M6 | 4% PFA | frozen | CSPG, |
List of used monkey cases providing information about experiments, fixation, sectioning and staining methods. ACAN, aggrecan; AChE, acetylcholinesterase; ChAT, choline acetyltransferase; CSPG, chondroitin sulfate proteoglycan; CTB, choleratoxin subunit B; DAB, diaminobenzidine; nIII, oculomotor nucleus; WGA, wheat germ agglutinin.