Table 2.

Human cases.

Case	Post-mortem delay	Fixation	Fixation duration	Section type	Staining methods
H1	24 h	4% PFA	2 days	paraffin	ChAT (DAB-Ni) + CSPG (DAB)
H2	24 h	4% PFA	6 days	paraffin	ChAT (DAB-Ni) + CSPG (DAB)
H3	24 h	4% PFA	6 days	paraffin	Nissl
H4	8 h	1% PFA/2.5% glutaraldehyde	12 h	vibratome	AChE enzyme histochemistry

List of used human cases providing information about post-mortem delay, fixation, sectioning and staining methods. AChE, acetylcholinesterase; ChAT, choline acetyltransferase; CSPG, chondroitin sulfate proteoglycan; CTB, choleratoxin subunit B; DAB, diaminobenzidine; DAB-Ni, nickel-enhanced DAB; WGA, wheat germ agglutinin.