FIGURE 1.

Schematic workflow of fecal microbiota transplantation (FMT) in pigs. At first, optimal donors should be selected to ensure the efficacy of porcine FMT and reduce the risks of transmitting infectious diseases during the transfer of fecal suspension via strict genetic backgrounds investigation, phenotypic characteristics, and serological test and stool testing. Fresh feces from healthy donor pigs were homogenized with sterile saline (0.9% NaCl) in blender and the stool materials should be diluted 3–5 times with large volumes of the buffer solution. The slurry was then filtered through sterile sieves and the suspension is either transferred to the recipients or mixed with 10% sterile glycerol to store at –80°C immediately. When there is the need for porcine FMT, the frozen fecal suspension should be thawed at 37°C (water bath).