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. 2018 Jun 19;9:820. doi: 10.3389/fpls.2018.00820

FIGURE 2.

FIGURE 2

Identification of the products of xanthotoxin metabolization by CYP71AZ1 (A,D) and CYP71AZ6 (B,E). Yeast microsomes expressing CYP71AZ1 or CYP71AZ6 convert xanthotoxin to 5-hydroxyxanthotoxin (P01 and P02). (A) HPLC profile of enzyme assays containing xanthotoxin, without (line 1) or with (line 2) NADPH, oxygen, and yeast microsomes expressing CYP71AZ1. (B) HPLC profile of enzyme assays containing xanthotoxin, without (line 1) or with (line 2) NADPH, oxygen, and yeast microsomes expressing CYP71AZ6. (C) MS and absorption spectrum of 5-hydroxyxanthotoxin standard. (D) MS and absorption spectrum of P01. (E) MS and absorption spectrum of P02.