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. 2018 Jun 19;9:820. doi: 10.3389/fpls.2018.00820

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Copyright © 2018 Krieger, Roselli, Kellner-Thielmann, Galati, Schneider, Grosjean, Olry, Ritchie, Matern, Bourgaud and Hehn.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of the product of esculetin metabolization by CYP71AZ3. Yeast microsomes expressing CYP71AZ3 convert esculetin to 6,7,8-trihydroxycoumarin (P13). (A) HPLC profile of enzyme assays containing esculetin, without (line 1) or with (line 2) NADPH, oxygen, and yeast microsomes expressing CYP71AZ3. (B) MS and absorption spectrum of standard 6,7,8-trihydroxycoumarin. (C) MS and absorption spectrum of P13.